Messenger RNA (mRNA) is a single-stranded RNA molecule that corresponds to the genetic sequence of a gene and is read by the ribosome in the process of producing a protein. mRNA is created during the process of transcription, where the enzyme RNA polymerase converts genes into primary transcript mRNA (also known as pre-mRNA). This pre-mRNA usually still contains introns, regions that will not go on to code for the final amino acid sequence. These are removed in the process of RNA splicing, leaving only exons, regions that will encode the protein. This exon sequence constitutes mature mRNA. Mature mRNA is then read by the ribosome, and, utilising amino acids carried by transfer RNA (tRNA), the ribosome creates the protein. This process is known as translation. All of these processes form part of the central dogma of molecular biology, which describes the flow of genetic information in a biological system.
Paints in powdered form require the addition of a liquid to prepare them for use. The manufacturer's directions as to the amount of oil, varnish, water, or other vehicle required should be followed. Fortunately, you can always get your primer tinted for free. Getting your primer colored the same as your paint will save you a coat of paint. Of course, I always recommend doing a minimum of 2 coats of paint so that your finish sheen looks consistent. Cheaper then doing it yourself. Some would argue that if you're going to all the trouble to fully prep the car yourself, then why not just shoot the paint as well? Why get a shop involved? The answer is simply that you'll get a better job and for less money. Some would argue with this, but the fact is that if you've never sprayed a car before then your first car will have a steep, and expensive learning curve. Laying down an even, consistent coat of paint takes considerable practice. Also, paint and equipment is not cheap. You'll need to buy primer, color coat, clear coat, reducers & catalysts. None of these are inexpensive and you can easily spend as much in paint materials alone as the cheapest paint job at Maaco or similar shop.
Also, when checking the paint, be realistic and don't ignore areas just for the sake of convenience. It will cost you more in time and money later. Typically if one area of the paint is cracked or peeling, then the paint on the entire car is suspect. Maybe the rest of the car just hasn't quite reached that point of decay, but it will shortly. So if you only fix areas that look bad, you'll likely find that you wasted a paint job when the other areas start to crack or peel in a year or two. Once the drywall compound/spackle has had sufficient time to dry, you will need to lightly sand the surface of the repair to smooth out any ridges that may have been caused while "coating" the patch. Once you have the surface sanded smooth, take a wet sponge or cloth and, by lightly washing/rubbing, blend the outside edge of the drywall compound/spackle back into the wall. For a smaller repair, you can wash away the excess drywall compound, leaving only the compound/spackle only in the area where the indent was filled. For a larger hole, after filling, sanding and using a wet sponge to blend the outside edge of the compound back into the wall, it may be necessary to texture the area in order to give the smooth patch the appearance of the existing wall. Today, it is increasingly easy to source ecologically sound paints, as most specialist ranges, such as Ecos, Earthborn, Georgina Barrow and Auro are available via mail order. There is a wealth of colour options in these pre-mixed ranges that include lush shades and muted palettes, reflecting their natural ingredients. Ecos continues to lead the field. It has recently developed Atmosphere Purifying Paint, which absorbs and neutralises volatile chemicals, solvents and VOCs from the atmosphere in a home. Selecting a Shop. You should always get quotes from at least 2-3 different shops near you. This will give you a chance to not only compare prices, but to also check out the quality of work each shop has done. Ask to see cars they've just painted. Look for orange peel or excessive overspray. Do they mostly do insurance work or do they paint entire cars also? What types of cars are they working on? A shop that sprays nothing but old beaters probably doesn't inspire as much confidence as one that does insurance work for the local Lexus or Mercedes dealer.